期刊
MOLECULAR BIOLOGY REPORTS
卷 37, 期 2, 页码 1137-1142出版社
SPRINGER
DOI: 10.1007/s11033-009-9886-7
关键词
Chrysanthemum; DgZFP; Gene expression; Salt stress; Cys(2)/His(2)-type zinc finger protein; Transgenic tobacco
资金
- National Post-doctoral Science Foundation [20060390096]
- [2006AA100109]
A Cys(2)/His(2)-type zinc finger protein gene, DgZFP, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach. The DgZFP encodes a protein of 211 amino acids residues with a calculated molecular mass of 22.9 kDa and theoretical isoelectric point is 8.59. DgZFP contains two Cys(2)/His(2)-type zinc finger motifs, one nuclear localization domain, one Leu-rich domain, and one ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression (EAR) domain. The transcript of DgZFP was enriched in flowers than in roots, stems, and leaves of the adult chrysanthemum plants. The gene expression was strongly induced by NaCl, drought and cold treatment, and weakly by ABA treatment in the seedlings. Subcellular localization revealed that DgZFP was localized preferentially distributed to nucleus. Overexpression of DgZFP improved salt tolerance and resulted in growth suppression in transgenic tobacco. We argued that DgZFP is a new member of the Cys(2)/His(2)-type zinc finger protein genes, and it maybe function as a regulator in response to salt stress in plants.
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