期刊
MOLECULAR BIOLOGY REPORTS
卷 37, 期 2, 页码 661-668出版社
SPRINGER
DOI: 10.1007/s11033-009-9510-x
关键词
Gene cloning; Gene expression; Rubisco activase; Ipomoea batatas (L.) Lam
A full-length cDNA of Rubisco activase (IBrcaI) was cloned from sweet potato (Ipomoea batatas (L.) Lam) using Rapid-Amplification of cDNA Ends (RACE). IBrcaI contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. The sequence alignment of multiple Rubisco activase genes from sweet potato and other plants showed high homology at two previously described ATP-binding sites. Western blot analysis indicated that there are two Rubisco activase proteins in sweet potato. Expression of IBrcaI was only detected in leaves. In the 14 h light and 10 h dark photoperiods, maximal and minimal IBrcaI mRNA expression levels were detected at 8:00 in the morning and at midnight, respectively.
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