期刊
MOLECULAR BIOLOGY REPORTS
卷 36, 期 4, 页码 711-716出版社
SPRINGER
DOI: 10.1007/s11033-008-9233-4
关键词
Anionic defensin-like gene; Bombyx mori; Expression pattern; Innate immunity; Sequence structure
资金
- Ministry of Education, Culture, Sports, Science, and Technology, Japan
- Japan Society for the Promotion of Science, Japan [18380041]
- The Fund for Foreign Scholars in University Research and Teaching Programs [B07045]
- Ministry of Education and State Administration of Foreign Experts Affairs, P. R. China
- Grants-in-Aid for Scientific Research [18380041] Funding Source: KAKEN
A defensin-like gene, BmdefA, was rediscovered in the silkworm genome and expressed sequence tags databases. The open reading frame of BmdefA encodes a prepropeptide consisting of a 22-residue signal peptide, a 34-residue propeptide, and a 36-residue mature peptide with a molecular mass of 4.0 kDa. The mature peptide possesses the characteristic six-cysteine motif of insect defensins, and its predicted isoelectric point is 4.12, indicating it is a novel anionic defensin. An intron is present in BmdefA and several cis-regulatory elements are in the regulating region. It is transcribed constitutively at a high level in the hemocyte, silk gland, head, and ovary of the silkworm larvae, and in the fat body of early-stage pupae and moth. BmdefA is also strongly induced by immune challenge. These results suggest that BmdefA plays an important role in both immunity and metamorphosis.
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