期刊
SCIENCE
卷 348, 期 6241, 页码 1369-1372出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa9555
关键词
-
资金
- Marie Curie Actions COFUND (European Commission Cofunding of Regional, National, and International Programme) [229597]
During clathrin-mediated endocytosis (CME), plasma membrane regions are internalized to retrieve extracellular molecules and cell surface components. Whether endocytosis occurs by direct clathrin assembly into curved lattices on the budding vesicle or by initial recruitment to flat membranes and subsequent reshaping has been controversial. To distinguish between these models, we combined fluorescence microscopy and electron tomography to locate endocytic sites and to determine their coat and membrane shapes during invagination. The curvature of the clathrin coat increased, whereas the coated surface area remained nearly constant. Furthermore, clathrin rapidly exchanged at all stages of CME. Thus, coated vesicle budding appears to involve bending of a dynamic preassembled clathrin coat.
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