期刊
MOLECULAR BIOLOGY OF THE CELL
卷 25, 期 7, 页码 1127-1136出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-08-0477
关键词
-
类别
资金
- Morasha Legacy [1798/10]
- Binational Israel-USA Science Foundation
- National Institutes of Health [RO1 GM048646]
- Israel Ministry of Health [MOH 2965]
- Muscular Dystrophy Association
- COST NANONET [BM1002]
Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Caenorhabditis elegans BAF-1 mobility is regulated by caloric restriction, food deprivation, and heat shock. This was not a general response of chromatin-associated proteins, as food deprivation did not affect the mobility of heterochromatin protein HPL-1 or HPL-2. Heat shock also increased the level of BAF-1 Ser-4 phosphorylation. By using missense mutations that affect BAF-1 binding to different partners we find that, overall, the ability of BAF-1 mutants to be immobilized by heat shock in intestinal cells correlated with normal or increased affinity for emerin in vitro. These results show BAF-1 localization and mobility at the nuclear lamina are regulated by stress and unexpectedly reveal BAF-1 immobilization as a specific response to caloric restriction in C. elegans intestinal cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据