期刊
MOLECULAR BIOLOGY OF THE CELL
卷 24, 期 15, 页码 2340-2349出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E12-10-0728
关键词
-
类别
资金
- Deutsche Forschungsgemeinschaft [SPP1474, FOR 929]
The maintenance of rod-cell shape in many bacteria depends on actin-like MreB proteins and several membrane proteins that interact with MreB. Using superresolution microscopy, we show that at 50-nm resolution, Bacillus subtilis MreB forms filamentous structures of length up to 3.4 mu m underneath the cell membrane, which run at angles diverging up to 40 degrees relative to the cell circumference. MreB from Escherichia coli forms at least 1.4-mu m-long filaments. MreB filaments move along various tracks with a maximal speed of 85 nm/s, and the loss of ATPase activity leads to the formation of extended and static filaments. Sub-optimal growth conditions lead to formation of patch-like structures rather than extended filaments. Coexpression of wild-type MreB with MreB mutated in the subunit interface leads to formation of shorter MreB filaments and a strong effect on cell shape, revealing a link between filament length and cell morphology. Thus MreB has an extended-filament architecture with the potential to position membrane proteins over long distances, whose localization in turn may affect the shape of the cell wall.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据