期刊
MOLECULAR BIOLOGY OF THE CELL
卷 24, 期 21, 页码 3406-3419出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-04-0185
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资金
- National Institutes of Health Grants [R01 GM061156, R01 GM071574]
- Grants-in-Aid for Scientific Research [24770189] Funding Source: KAKEN
During the budding of coat protein complex II (COPII) vesicles from transitional endoplasmic reticulum (tER) sites, Sec16 has been proposed to play two distinct roles: negatively regulating COPII turnover and organizing COPII assembly at tER sites. We tested these ideas using the yeast Pichia pastoris. Redistribution of Sec16 to the cytosol accelerates tER dynamics, supporting a negative regulatory role for Sec16. To evaluate a possible COPII organization role, we dissected the functional regions of Sec16. The central conserved domain, which had been implicated in coordinating COPII assembly, is actually dispensable for normal tER structure. An upstream conserved region (UCR) localizes Sec16 to tER sites. The UCR binds COPII components, and removal of COPII from tER sites also removes Sec16, indicating that COPII recruits Sec16 rather than the other way around. We propose that Sec16 does not in fact organize COPII. Instead, regulation of COPII turnover can account for the influence of Sec16 on tER sites.
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