期刊
MOLECULAR BIOLOGY OF THE CELL
卷 24, 期 5, 页码 535-542出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E12-09-0677
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A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.
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