期刊
MOLECULAR BIOLOGY OF THE CELL
卷 23, 期 16, 页码 3143-3155出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E12-05-0357
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资金
- National Institutes of Health [GM083122, GM66919]
- W. M. Keck Foundation
- National Science Foundation [DMR-MRSEC-0820492]
- Pew Biomedical Scholars Award
Motile cilia and flagella are highly conserved organelles that play important roles in human health and development. We recently discovered a calmodulin-and spoke-associated complex (CSC) that is required for wild-type motility and for the stable assembly of a subset of radial spokes. Using cryo-electron tomography, we present the first structure-based localization model of the CSC. Chlamydomonas flagella have two full-length radial spokes, RS1 and RS2, and a shorter RS3 homologue, the RS3 stand-in (RS3S). Using newly developed techniques for analyzing samples with structural heterogeneity, we demonstrate that the CSC connects three major axonemal complexes involved in dynein regulation: RS2, the nexin-dynein regulatory complex (N-DRC), and RS3S. These results provide insights into how signals from the radial spokes may be transmitted to the N-DRC and ultimately to the dynein motors. Our results also indicate that although structurally very similar, RS1 and RS2 likely serve different functions in regulating flagellar motility.
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