期刊
MOLECULAR BIOLOGY OF THE CELL
卷 23, 期 13, 页码 2424-2432出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E10-12-0950
关键词
-
类别
资金
- Academy of Finland [131650]
- Biocenter Finland
- Emil Aaltonen Foundation
- Academy of Finland (AKA) [131650, 131650] Funding Source: Academy of Finland (AKA)
The endoplasmic reticulum (ER) is both structurally and functionally complex, consisting of a dynamic network of interconnected sheets and tubules. To achieve a more comprehensive view of ER organization in interphase and mitotic cells and to address a discrepancy in the field (i.e., whether ER sheets persist, or are transformed to tubules, during mitosis), we analyzed the ER in four different mammalian cell lines using live-cell imaging, high-resolution electron microscopy, and three dimensional electron microscopy. In interphase cells, we found great variation in network organization and sheet structures among different cell lines. In mitotic cells, we show that the ER undergoes both spatial reorganization and structural transformation of sheets toward more fenestrated and tubular forms. However, the extent of spatial reorganization and sheet-to-tubule transformation varies among cell lines. Fenestration and tubulation of the ER correlates with a reduced number of membrane-bound ribosomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据