期刊
MOLECULAR BIOLOGY OF THE CELL
卷 23, 期 16, 页码 3203-3214出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E12-01-0034
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Extreme Photonics and the Cellular Systems Biology Projects of RIKEN
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [23012010, 11J09001, 24114003] Funding Source: KAKEN
The Golgi apparatus forms stacks of cisternae in many eukaryotic cells. However, little is known about how such a stacked structure is formed and maintained. To address this question, plant cells provide a system suitable for live-imaging approaches because individual Golgi stacks are well separated in the cytoplasm. We established tobacco BY-2 cell lines expressing multiple Golgi markers tagged by different fluorescent proteins and observed their responses to brefeldin A (BFA) treatment and BFA removal. BFA treatment disrupted cis, medial, and trans cisternae but caused distinct relocalization patterns depending on the proteins examined. Medial-and trans-Golgi proteins, as well as one cis-Golgi protein, were absorbed into the endoplasmic reticulum (ER), but two other cis-Golgi proteins formed small punctate structures. After BFA removal, these puncta coalesced first, and then the Golgi stacks regenerated from them in the cis-to-trans order. We suggest that these structures have a property similar to the ER-Golgi intermediate compartment and function as the scaffold of Golgi regeneration.
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