期刊
SCIENCE
卷 349, 期 6245, 页码 324-328出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1260031
关键词
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资金
- Air Force Office of Scientific Research [13RSL012]
- NIH [GM107521, GM100116, GM062419, AI081982, AI101436]
- Burroughs-Wellcome Career Award at the Scientific Interface
- American Cancer Society Postdoctoral Fellowship [PF-12-262-01-MPC]
- NSF
- EPSRC [EP/D048559/1, EP/L011972/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/D048559/1, EP/L011972/1] Funding Source: researchfish
Organisms are adapted to the relentless cycles of day and night, because they evolved timekeeping systems called circadian clocks, which regulate biological activities with similar to 24-hour rhythms. The clock of cyanobacteria is driven by a three-protein oscillator composed of KaiA, KaiB, and KaiC, which together generate a circadian rhythm of KaiC phosphorylation. We show that KaiB flips between two distinct three-dimensional folds, and its rare transition to an active state provides a time delay that is required to match the timing of the oscillator to that of Earth's rotation. Once KaiB switches folds, it binds phosphorylated KaiC and captures KaiA, which initiates a phase transition of the circadian cycle, and it regulates components of the clock-output pathway, which provides the link that joins the timekeeping and signaling functions of the oscillator.
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