期刊
MOLECULAR BIOLOGY OF THE CELL
卷 22, 期 4, 页码 448-456出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E10-03-0203
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资金
- National Institutes of Health (NIH) [R01CA126832, R01GM081466]
- Welch Foundation [I-1594]
Dynamic instability is a critical property of microtubules (MTs). By regulating the rate of tubulin polymerization and depolymerization, cells organize the MT cytoskeleton to accommodate their specific functions. Among many processes, posttranslational modifications of tubulin are implicated in regulating MT functions. Here we report a novel tubulin acetylation catalyzed by acetyltransferase San at lysine 252 (K252) of beta-tubulin. This acetylation, which is also detected in vivo, is added to soluble tubulin heterodimers but not tubulins in MTs. The acetylation-mimicking K252A/Q mutants were incorporated into the MT cytoskeleton in HeLa cells without causing any obvious MT defect. However, after cold-induced catastrophe, MT regrowth is accelerated in San-siRNA cells while the incorporation of acetylation-mimicking mutant tubulins is severely impeded. K252 of alpha-tubulin localizes at the interface of alpha-/beta-tubulins and interacts with the phosphate group of the beta-tubulin-bound GTP. We propose that the acetylation slows down tubulin incorporation into MTs by neutralizing the positive charge on K252 and allowing tubulin heterodimers to adopt a conformation that disfavors tubulin incorporation.
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