期刊
MOLECULAR BIOLOGY OF THE CELL
卷 22, 期 23, 页码 4549-4562出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E11-05-0405
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资金
- Centre National de la Recherche Scientifique [UMR 6216]
- Universite de la Mediterranee
- European Community [HEALTH-F2-2008-200234]
- Agence Nationale de la Recherche (ANR) [BLAN07-2-186738]
- Ligue Nationale Contre le Cancer (LNCC)
- Fondation pour la Recherche Medicale
- ANR [BLAN07-2-186738]
- Marie Curie International Reintegration [46575]
- European Consortium for Anticancer Antibody Development [Health-F5-2008-200755]
- French Ministry for Research and Education
- Infrastructures en Biologie Sante et Agronomie (Plateforme de Proteomique, Marseille)
- Welch Foundation [I-1300]
- National Institutes of Health [EY10199]
Primary cilia originate from the centrosome and play essential roles in several cellular, developmental, and pathological processes, but the underlying mechanisms of ciliogenesis are not fully understood. Given the involvement of the adaptor protein Hook2 in centrosomal homeostasis and protein transport to pericentrosomal aggresomes, we explored its role in ciliogenesis. We found that in human retinal epithelial cells, Hook2 localizes at the Golgi apparatus and centrosome/basal body, a strategic partitioning for ciliogenesis. Of importance, Hook2 depletion disrupts ciliogenesis at a stage before the formation of the ciliary vesicle at the distal tip of the mother centriole. Using two hybrid and immunoprecipitation assays and a small interfering RNA strategy, we found that Hook2 interacts with and stabilizes pericentriolar material protein 1 (PCM1), which was reported to be essential for the recruitment of Rab8a, a GTPase that is believed to be crucial for membrane transport to the primary cilium. Of interest, GFP::Rab8a coimmunoprecipitates with endogenous Hook2 and PCM1. Finally, GFP::Rab8a can overcome Hook2 depletion, demonstrating a functional interaction between Hook2 and these two important regulators of ciliogenesis. The data indicate that Hook2 interacts with PCM1 in a complex that also contains Rab8a and regulates a limiting step required for further initiation of ciliogenesis after centriole maturation.
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