期刊
SCIENCE
卷 347, 期 6228, 页码 1367-1371出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa3380
关键词
-
资金
- Novartis Research Foundation
- NIH [NS83085, EB013571, GM57071]
- Howard Hughes Medical Institute
- European Molecular Biology Laboratory (EMBL)
- EMBL Interdisciplinary Postdoc Program (EIPOD) under Marie Curie COFUND actions
Analysis of single molecules in living cells has provided quantitative insights into the kinetics of fundamental biological processes; however, the dynamics of messenger RNA (mRNA) translation have yet to be addressed. We have developed a fluorescence microscopy technique that reports on the first translation events of individual mRNA molecules. This allowed us to examine the spatiotemporal regulation of translation during normal growth and stress and during Drosophila oocyte development. We have shown that mRNAs are not translated in the nucleus but translate within minutes after export, that sequestration within P-bodies regulates translation, and that oskar mRNA is not translated until it reaches the posterior pole of the oocyte. This methodology provides a framework for studying initiation of protein synthesis on single mRNAs in living cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据