期刊
MOLECULAR BIOLOGY OF THE CELL
卷 22, 期 13, 页码 2348-2359出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E09-12-1064
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资金
- National Institutes of Health [DK51596]
Previous studies have shown that membrane tubule-mediated export from endosomal compartments requires a cytoplasmic phospholipase A(2) (PLA(2)) activity. Here we report that the cytoplasmic PLA(2) enzyme complex platelet-activating factor acetylhydrolase (PAFAH) Ib, which consists of alpha 1, alpha 2, and LIS1 subunits, regulates the distribution and function of endosomes. The catalytic subunits alpha 1 and alpha 2 are located on early-sorting endosomes and the central endocytic recycling compartment (ERC) and their overexpression, but not overexpression of their catalytically inactive counterparts, induced endosome membrane tubules. In addition, overexpression alpha 1 and alpha 2 altered normal endocytic trafficking; transferrin was recycled back to the plasma membrane directly from peripheral early-sorting endosomes instead of making an intermediate stop in the ERC. Consistent with these results, small interfering RNA-mediated knockdown of alpha 1 and alpha 2 significantly inhibited the formation of endosome membrane tubules and delayed the recycling of transferrin. In addition, the results agree with previous reports that PAFAH Ib alpha 1 and alpha 2 expression levels affect the distribution of endosomes within the cell through interactions with the dynein regulator LIS1. These studies show that PAFAH Ib regulates endocytic membrane trafficking through novel mechanisms involving both PLA(2) activity and LIS1-dependent dynein function.
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