期刊
SCIENCE
卷 349, 期 6251, 页码 986-989出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa3794
关键词
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资金
- National Institutes of Health through NSF-National Institute of General Medical Sciences Mathematical Biology Program [R01GM104974]
- Robert A. Welch Foundation [C-1729]
- Hamill Foundation
- NSF [DMS-0931642]
- China Scholarship Council
A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types-an activator strain and a repressor strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types.
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