期刊
MOLECULAR BIOLOGY OF THE CELL
卷 20, 期 19, 页码 4194-4204出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E09-06-0489
关键词
-
类别
资金
- Darwin Trust of Edinburgh
- Wellcome Trust
- National Institutes of Health
- National Cancer Institute
- Center for Cancer Research
- Ministry of Education, Science, Sports and Culture of Japan
- Ministerio de Ciencia y Tecnologia [SAF2007-61863]
- MEXT of Japan
We previously used a human artificial chromosome ( HAC) with a synthetic kinetochore that could be targeted with chromatin modifiers fused to tetracycline repressor to show that targeting of the transcriptional repressor tTS within kinetochore chromatin disrupts kinetochore structure and function. Here we show that the transcriptional corepressor KAP1, a downstream effector of the tTS, can also inactivate the kinetochore. The disruption of kinetochore structure by KAP1 subdomains does not simply result from loss of centromeric CENP-A nucleosomes. Instead it reflects a hierarchical disruption of the outer kinetochore, with CENP-C levels falling before CENP-A levels and, in certain instances, CENP-H being lost more readily than CENP-C. These results suggest that this novel approach to kinetochore dissection may reveal new patterns of protein interactions within the kinetochore.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据