期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 31, 期 5, 页码 1272-1274出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msu063
关键词
phylogenomics; next-generation sequencing; restriction-site associated DNA sequencing; empirical data; Insecta; Carabidae
资金
- division Plant Health and the Environment of the French National Institute for Agricultural Research (INRA)
- network Library of Life by the National Center for Scientific Research (CNRS)
- National Museum of Natural History (MNHN)
- French National Agronomic Institute (INRA)
- French Alternative Energies and Atomic Energy Commission (CEA) (Genoscope)
Next-generation sequencing opened up new possibilities in phylogenetics; however, choosing an appropriate method of sample preparation remains challenging. Here, we demonstrate that restriction-site-associated DNA sequencing (RAD-seq) generates useful data for phylogenomics. Analysis of our RAD library using current bioinformatic and phylogenetic tools produced 400x more sites than our Sanger approach (2,262,825 nt/species), fully resolving relationships between 18 species of ground beetles (divergences up to 17 My). This suggests that RAD-seq is promising to infer phylogeny of eukaryotic species, though potential biases need to be evaluated and new methodologies developed to take full advantage of such data.
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