期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 26, 期 11, 页码 2617-2626出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msp180
关键词
insertion frequencies; haplotype; HERV-K113 and HERV-K115; hervotype; human evolution; Homo erectus
资金
- UCSF AIDS Research Institute
- Pfizer, Inc.,
- National Institutes of Health [1KO1DA024654, A176059]
- University of California San Francisco AIDS Research Institute
The human genome, human endogenous retroviruses (HERV), of which HERV-K 113 and HERV-K 115 are the only known full-length proviruses that are insertionally polymorphic. Although a handful of previously published papers have documented their prevalence in the global population; to date, there has been no report on their prevalence in the United States population. Here, we studied the geographic distribution of K113 and K115 among 156 HIV-1+ subjects from the United States, including African Americans, Hispanics, and Caucasians. In the individuals studied, we found higher insertion frequencies of K113 (21%) and K115 (35%) in African Americans compared with Caucasians (K113 9% and K115 6%) within the United States. We also report the presence of three single nucleotide polymorphism sites in the K113 5' long terminal repeats (LTRs) and four in the K115 5' LTR that together constituted four haplotypes for K113 Lind five haplotypes for K115. HERV insertion times can be estimated from the sequence differences between the 5' and 3' LTR of each insertion, but this dating method cannot be used with HERV-K115. We developed a method to estimate insertion times by applying coalescent inference to 5' LTR sequences within our study population and validated this approach using an independent estimate derived from the genetic distance between K113 5' and 3' LTR sequences. Using our method, we estimated the insertion dates of K113 and K115 to be it minimum of 800,000 and 1.1 million years ago, respectively. Both these insertion dates predate the emergence of anatomically modern Homo sapiens.
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