期刊
MOLECULAR BIOLOGY
卷 45, 期 4, 页码 647-657出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026893311030113
关键词
glycoside hydrolase; endo-alpha-1,4-polygalactosaminidase; GH114 family; COG2342 family; GH36 family; GHL families; new protein families; PSI-BLAST; PSI Protein Classifier; CAZy; TIM-barrel fold; protein phylogenetic tree; hierarchical protein classification; protein evolution; search for homologs; multiple sequence alignment; horizontal transfer; gene annotation
Endo-alpha-1,4-polygalactosaminidase is a rare enzyme. Its catalytic domain belongs to the GH114 family of glycoside hydrolases. It is shown by phylogenetic analysis that the evolution of the corresponding genes involved duplications, elimination, and horizontal transfer. The domain and secondary structures of endo-alpha-1,4-polygalactosaminidases are discussed. A hypothesis is put forward as to the structure of the active center of the enzyme. Iterative screening of a protein database reveals evolutionary relationships of the GH114 family with the GH13, GH18, GH20, GH27, GH29, GH31, GH35, GH36, and GH66 families of glycoside hydrolases and with the COG1306, COG1649, COG2342, GHL3, and GHL4 families of proteins with unknown enzymatic functions. Unclassified homologs are grouped into 13 new families of hypothetical glycoside hydrolases: GHL5-GHL15, GH36J, and GH36K.
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