期刊
SCIENCE
卷 348, 期 6241, 页码 1361-1365出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa5264
关键词
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资金
- NIH [R01GM083118, R01EY05216]
- Jules Stein Professor Endowment
- National Eye Institute Core Grant [P30EY00331]
- Research to Prevent Blindness
- German Academic Exchange Service (DAAD)
G protein-coupled receptors (GPCRs) relay diverse extracellular signals into cells by catalyzing nucleotide release from heterotrimeric G proteins, but the mechanism underlying this quintessential molecular signaling event has remained unclear. Here we use atomic-level simulations to elucidate the nucleotide-release mechanism. We find that the G protein a subunit Ras and helical domains-previously observed to separate widely upon receptor binding to expose the nucleotide-binding site-separate spontaneously and frequently even in the absence of a receptor. Domain separation is necessary but not sufficient for rapid nucleotide release. Rather, receptors catalyze nucleotide release by favoring an internal structural rearrangement of the Ras domain that weakens its nucleotide affinity. We use double electron-electron resonance spectroscopy and protein engineering to confirm predictions of our computationally determined mechanism.
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