期刊
MOLECULAR AND CELLULAR PROBES
卷 22, 期 4, 页码 238-243出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2008.04.002
关键词
Salmonella; Microarray; Oligonucleotide
类别
资金
- National Institute of Allergy and Infectious Diseases, National Institute of Health, Department of Health and Human Services [N01-AI-30054, ZC002-03]
- Cornell University Agricultural Experiment Station
We describe the development of a spotted array for the delineation of the most common 14 disease-causing Salmonella serovars in the United States. Our array consists of 414 70 mers targeting core genes of Salmonella enterica, subspecies I specific genes, fimbrial genes, pathogenicity islands, Gifsy elements and other variable genes. Using this array we were able to identify a unique gene presence/absence profile for each of the targeted serovar which was used as the serovar differentiating criteria. Based on this profile, we developed a Matlab programme that compares the profile of an unknown sample to all 14 reference serovar profiles and give out the closest serovar match. Since we have included probes targeting most of the virulence genes and variable genes in Salmonella, in addition to using for serovar detection this array could also be used for studying the virulence gene content and also for evaluating the genetic relation between different isolates of Salmonella. (C) 2008 Elsevier Ltd. All rights reserved.
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