期刊
SCIENCE
卷 350, 期 6266, 页码 1361-1366出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aab0810
关键词
-
资金
- Defense Advanced Research Projects Agency
- NIH Pioneer award
- NIH BRAIN Initiative U01 grant
- Stanford CNC Program
Genetically encoded voltage indicators (GEVIs) are a promising technology for fluorescence readout of millisecond-scale neuronal dynamics. Previous GEVIs had insufficient signaling speed and dynamic range to resolve action potentials in live animals. We coupled fast voltage-sensing domains from a rhodopsin protein to bright fluorophores through resonance energy transfer. The resulting GEVIs are sufficiently bright and fast to report neuronal action potentials and membrane voltage dynamics in awake mice and flies, resolving fast spike trains with 0.2-millisecond timing precision at spike detection error rates orders of magnitude better than previous GEVIs. In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation. These results empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.
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