期刊
SCIENCE
卷 348, 期 6239, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa0698
关键词
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资金
- NIH [R37AI067073, N01-AI-30024, N01-AI-15422, N01-A1-30024]
- International AIDS Vaccine Initiative [UKZNRSA1001]
- South African Research Chairs Initiative
- Victor Daitz Foundation
- International Early Career Scientist Award from the Howard Hughes Medical Institute
- NIH-National Institute of Dental and Craniofacial Research [R01 DE018925-04]
- HIVACAT program
- CUTHIVAC [241904]
- TRF Senior Research Scholar, the Thailand Research Fund
- Chulalongkorn University Research Professor Program, Thailand
- NSF Graduate Research Fellowships Program
- [NIH DA033541]
- [AI082630]
- ICREA Funding Source: Custom
The human virome plays important roles in health and immunity. However, current methods for detecting viral infections and antiviral responses have limited throughput and coverage. Here, we present VirScan, a high-throughput method to comprehensively analyze antiviral antibodies using immunoprecipitation and massively parallel DNA sequencing of a bacteriophage library displaying proteome-wide peptides from all human viruses. We assayed over 108 antibody-peptide interactions in 569 humans across four continents, nearly doubling the number of previously established viral epitopes. We detected antibodies to an average of 10 viral species per person and 84 species in at least two individuals. Although rates of specific virus exposure were heterogeneous across populations, antibody responses targeted strongly conserved public epitopes for each virus, suggesting that they may elicit highly similar antibodies. VirScan is a powerful approach for studying interactions between the virome and the immune system.
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