期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 43, 期 1, 页码 108-116出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2009.09.012
关键词
Ras; Rin1; Gene expression; Telencephalon; REST; Snail
资金
- NIH [R01 NS046787, RO1 MH077022]
- UCLA Chancellor's Bioscience Initiative
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH077022] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS046787] Funding Source: NIH RePORTER
The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snail was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons. (C) 2009 Elsevier Inc. All rights reserved.
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