期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 41, 期 4, 页码 397-408出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2009.04.004
关键词
Fibronectin type III domains; Growth cone; Neurite outgrowth; Hippocampal neurons; Tenascin glycoprotein; Ig superfamily; beta 1 integrin; IP3; SERCA; Ryanodine receptor; Cell adhesion molecules; Protein kinase C; Calmodulin kinase
资金
- German Research Council (DFG) [642/A9]
Tenascin-C (Tnc) is transiently expressed during neural development. Within its alternatively spliced fibronectin type III (TNfn) -motifs the TNfnD domain is Crucial for a neurite outgrowth-promoting region that is recognized by the GPI-linked adhesion molecule of the Ig-superfamily contactin. In order to understand the downstream signaling mechanisms, embryonic day E18 rat hippocampal neurons were cultivated on TNfnBD-containing and control substrates in the presence of various inhibitors. As predicted, axon outgrowth promotion Could be Suppressed by antibodies to the TNfnD domain, to contactin, or to the beta 1-integrin subunit. The chelators BAPTA/AM or EGTA as well as blockade of membrane-based Calcium channels or of the release of calcium from intracellular stores reduced axon growth to control levels. The inhibition of phospholipase C and its downstream targets protein kinase C or calmodulin kinase likewise blocked outgrowth promotion. We propose that TNfnBD stimulates the Outgrowth of hippocampal neurons by activating calcium- and phospholipase C-depending pathways. Digital video microscopy Studies revealed that increase of fiber length was caused by an augmentation of growth cone velocity. (c) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据