期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 37, 期 1, 页码 69-84出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2007.08.015
关键词
gonadotropin-releasing hormone 1 (GnRH1); heterogeneous nuclear ribonucleoprotein A/B (hnRNP-A/B); heterogeneous nuclear ribonucleoprotein G (hnRNP-G); transcriptional regulation; mass spectrometric peptide mapping; hypothalamic-pituitary-gonadal (HPG) axis
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS034950, R56NS034950, R37NS034950] Funding Source: NIH RePORTER
- NINDS NIH HHS [R01 NS034950, R56 NS034950, NS 34950, R37 NS034950, R56 NS034950-16A1, R37 NS034950-15] Funding Source: Medline
Gonadotropin-releasing hormone 1 (GnRH1) causes the release of gonadotropins from the pituitary to control reproduction. Here we report that two heterogeneous nuclear ribonucleoproteins (hnRNP-A/B and hnRNP-G) bind to the GnRH-I upstream promoter region in a cichlid fish Astatotilapia burtoni. We identified these binding proteins using a newly developed homology based method of mass spectrometric peptide mapping. We show that both hnRNP-A/B and hnRNP-G colocalize with GnRH1 in the pre-optic area of the hypothalamus in the brain. We also demonstrated that these ribonucleoproteins exhibit similar binding capacity in vivo, using immortalized mouse GT1-7 cells where overexpression of either hnRNP-A/B or hnRNP-G significantly down-regulates GnRH1 mRNA levels in GT1-7 cells, suggesting that both act as repressors in GnRH1 transcriptional regulation. (C) 2007 Elsevier Inc. All rights reserved.
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