期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 393, 期 1-2, 页码 129-142出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2014.06.010
关键词
Fatty acid; Tumour necrosis factor-alpha; p38 Mitogen-activated protein kinase; Insulin resistance; Skeletal muscle; Macrophage
资金
- Biotechnology and Biological Sciences Research Council (UK) Doctoral Training Grant PhD Scholarship
- Royal Society
- Wellcome Trust University Award [087461]
Obesity and saturated fatty acid (SFA) treatment are both associated with skeletal muscle insulin resistance (IR) and increased macrophage infiltration. However, the relative effects of SFA and unsaturated fatty acid (UFA)-activated macrophages on muscle are unknown. Here, macrophages were treated with palmitic acid, palmitoleic acid or both and the effects of the conditioned medium (CM) on C2C12 myotubes investigated. CM from palmitic acid-treated J774s (palm-mac-CM) impaired insulin signalling and insulin-stimulated glycogen synthesis, reduced Inhibitor kappa B alpha and increased phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase in myotubes. p38 MAPK inhibition or siRNA partially ameliorated these defects, as, did addition of tumour necrosis factor-a blocking antibody to the CM. Macrophages incubated with both FAs generated CM that did not induce IR, while palmitoleic acid-mac-CM alone was insulin sensitising. Thus UFAs may improve muscle insulin sensitivity and counteract SFA-mediated IR through an effect on macrophage activation. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据