期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 383, 期 1-2, 页码 159-169出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.12.011
关键词
Endocannabinoids; GPR55; CBI; White adipose tissue; LPI; Nutritional status
资金
- Ministerio de Economia y Competitividad [CD: BFU2011, RN: RYC-2008-02219, BFU2012-35255, MMM: BFU2010-17116]
- Xunta de Galicia [ML: 10PXIB208164PR, 2012-CP070, RN: EM 2012/039, 2012-CP069]
- Fondo de Investigaciones Sanitarias [ML: PI12/ 01814]
- Centro de Investigacion Biomedica en Red (CIBER) de Fisiopatologia de la Obesidad y Nutricion (CIBERobn)
- FEDER funds
- European Community's Seventh Framework Programme [245009]
The G protein-coupled receptor GPR55 has been proposed as a new cannabinoid receptor associated with obesity in humans. We have investigated the regulation of GPR55 in rat white adipose tissue (WAT) in different physiological and pathophysiological settings involved in energy balance. We compared GPR55 expression with Cannabinoid Receptor type I (CBI), which mediates the metabolic actions of endocannabinoids, by real time PCR and western blotting. Circulating levels of lysophosphatidylinositol (LPI), the endogenous ligand of GPR55, were measured by liquid chromatography-mass spectrometry. Both WAT CBI and GPR55 levels were increased after fasting and recovered after leptin treatment. Their expression was decreased during gestation and increased throughout lifespan. Orchidectomy diminished WAT CBI and GPR55 expression whereas ovariectomized rats showed increased GPR55 but decreased CBI levels. Alterations in pituitary functions also modified WAT CBI and GPR55 levels. Serum LPI levels were inversely regulated by fasting and gonadectomy in comparison to WAT GPR55. Our findings indicate that GPR55 and LPI are regulated by different physiological and pathophysiological settings known to be associated with marked alterations in energy status. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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