期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 374, 期 1-2, 页码 65-72出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.04.008
关键词
Spexin; LH; Reproduction; Neuropeptide
资金
- National Basic Research Program (973 program) [2010CB126302]
- Hong Kong Research Grants Council [462409]
- National Science Foundation of China [31072196, 31101886, 31261160493]
- Guangdong Provincial Natural Science Foundation [9151027501000081, S20110400016030]
- Guangdong Provincial Science and Technology Program [2012B090500008]
- China Postdoctoral Science Foundation [343 20100480815]
- project of Science and Technology new star in Zhujiang city [2013-77]
- Fundamental Research Funds for the Central Universities [10lgpy21, 131gpy17]
The fish reproductive axis is regulated by many neuroendocrine factors. However, factors involved in the suppression of this axis are largely uncharacterized. In this study, we describe a novel neuropeptide derived from the spexin precursor acting as a negative factor to suppress the reproductive axis in teleost. The cDNA sequences of the spexin precursors have been cloned from both zebrafish and goldfish. A 14-aa mature peptide with the C-terminal amidated (spexin-14a: NWTPQAMLYLKGTQ-NH2) is conceivably generated by processing of the spexin precursors in both species. Spexin is mainly expressed in the brain and ovary of zebrafish and spexin-14a-ir cells are located in several brain regions of goldfish. Functionally, goldfish spexin-14a could significantly suppress luteinizing hormone (LH) release in cultured goldfish pituitary cells. Moreover, intraperitoneal injection of spexin-14a could effectively suppress serum LH level. The mRNA expression of spexin is lower in the breeding season and hypothalamic expression of spexin is regulated by gonadal hormones. These results constitute the first report on the novel role of spexin in the negative regulation of the reproductive axis in teleost (c) 2013 Elsevier Ireland Ltd. All rights reserved.
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