期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 370, 期 1-2, 页码 32-41出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.02.010
关键词
Gonadotropins; Germ cell; Meiotic initiation; Mitosis; MiR181a*; Chicken
资金
- National Natural Science Foundation of China [31272525]
- Zhejiang Provincial Natural Science Foundation [Z3110115]
- Fundamental Research Funds for the Central Universities
Gonadotropins are required for gametogenesis but in embryonic gonads this mechanism is not well understood. Here we use chicken embryos to investigate the mechanism that gonadotropins regulate the ovarian germ cell mitosis/meiosis decision. Treatment with follicle-stimulating hormone (FSH) delayed germ cell meiosis entry and promoted their proliferation. This action was blocked by an aromatase inhibitor. Treatment with luteinizing hormone (LH) accelerated germ cell meiosis entry and promoted transcription of 3 beta HSDII to increase progesterone (P-4) production. In the cultured ovaries, P-4 triggered meiotic initiation in germ cells. MiR181a*, which acts to downregulate the NR6A1 transcript to inhibit the meiotic initiation, was upregulated by FSH and downregulated by LH. Collectively, gonadotropins regulate germ cells mitosis and meiotic initiation through steroid hormones and a miR181a*-mediated pathway. In particularly, FSH delays germ cell meiosis entry and promotes cell proliferation via estrogen while LH accelerates the meiotic initiation via elevated P4 production. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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