期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 350, 期 1, 页码 39-52出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2011.11.019
关键词
Transforming growth factor-beta; Follistatin; Myostatin; Myosin heavy chain II
资金
- National Institute of Aging [SC1AG033407]
- Charles Drew University MSI [5S21MD000103]
- [1SC1CA165865-01A1]
- [5U54CA 143931]
Testosterone (T) administration is associated with increased satellite cell number and skeletal muscle hypertrophy, although there is considerable heterogeneity in the response of different skeletal muscle groups to T in vivo. We investigated the effects of T on the growth and differentiation of satellite cells isolated from levator ani (LA) and gastrocnemius (gastroc) muscles. T up regulated follistatin (Fst) expression, but down regulated the mRNA and protein expression of a number of genes in the transforming growth factor-beta (TGF-beta)-signaling pathway. Inhibition of Fst expression by small interfering RNA (siRNA) inhibited myogenic differentiation and blocked the pro-myogenic effects of T. Treatment of satellite cells with T or Fst up regulated the expression of Pax7 and PCNA, and increased their proliferation. T and Fst blocked TGF-beta induced inhibition of growth and myogenic differentiation and down regulated TGF-beta-dependent transcriptome in both LA and gastroc cells. We conclude that T stimulation of satellite cell proliferation and myogenic differentiation are associated with up regulation of Fst and inhibition of TGF-beta-signaling. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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