期刊
SCIENCE
卷 347, 期 6228, 页码 1371-1374出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1258849
关键词
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资金
- National Science Foundation [PHY 082265, PHY 1147498]
- National Institutes of Health [GM 112659, GM065367, GM082837, GM092830]
- Welch Foundation [Q-1759]
- Jane Coffin Childs Memorial Fund for Medical Research
- Division Of Physics
- Direct For Mathematical & Physical Scien [1430124] Funding Source: National Science Foundation
- Division Of Physics
- Direct For Mathematical & Physical Scien [1147498] Funding Source: National Science Foundation
Base-pairing interactions between nucleic acids mediate target recognition in many biological processes. We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics. We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs. This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.
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