4.5 Article

Transgenic mice expressing the human growth hormone gene provide a model system to study human growth hormone synthesis and secretion in non-tumor-derived pituitary cells: Differential effects of dexamethasone and thyroid hormone

期刊

MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 345, 期 1-2, 页码 48-57

出版社

ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2011.07.010

关键词

Human growth hormone; Dexamethasone; Thyroid hormone; Pituitary; Primary cultures; Transgenic mice

资金

  1. Manitoba Institute of Child Health
  2. Manitoba Health Research Council
  3. Canadian Institutes of Health Research [MT-10853]

向作者/读者索取更多资源

Growth hormone (GH) is regulated by pituitary and hypothalamic factors as well as peripheral endocrine factors including glucocorticoids and thyroid hormone. Studies on human GH are limited largely to the assessment of plasma levels in endocrine disorders. Thus, insight into the regulation of synthesis versus secretion has come mainly from studies done on non-human GH and/or pituitary tumor cells. However, primate and non-primate GH gene loci have differences in their structure and, by extension, regulation. We generated transgenic (171hGH/CS-TG) mice containing the intact hGH1 gene and locus control region, including sequences required for integration-independent and preferential pituitary expression. Here, we show hGH co-localizes with mouse (m) GH in somatotrophs in situ and in primary pituitary cells. Dexamethasone treatment increased hGH and mGH, as well as GH releasing hormone (GHRH) receptor RNA levels, and hGH release was stimulated by GHRH treatment. By contrast, triiodothyronine decreased or had no effect on hGH and mGH production, respectively, and the negative effect on hGH was also seen in the presence of dexamethasone. Thus, 171hGH/CS-TG mouse pituitary cultures represent a model system to investigate hormonal control of hGH synthesis and secretion. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

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