期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 333, 期 2, 页码 134-142出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2010.12.021
关键词
Spermatogenesis; Nucleocytoplasmic transport; XY body; Meiotic sex chromosome inactivation; Manchette; Meiosis; Spermiogenesis
资金
- NIH [HD050281, HD060858]
- University of Nevada, Reno
- Shanghai Leading Academic Discipline Project [S30201]
- Science and Technology Commission of Shanghai Municipality [10DZ2270600]
- Chinese Scholarship Council
We identified Ran-binding protein 17 (RANBP17) as one of the interacting partners of sperm maturation 1 (SPEM1) using yeast 2-hybrid screening and immunoprecipitation assays. Expression profiling analyses suggested that RANBP17 was preferentially expressed in the testis. Immunofluorescent confocal microscopy revealed a dynamic localization pattern of RANBP17 during spermatogenesis. In primary spermatocytes RANBP17 was mainly localized to the XY body. In the subsequent spermiogenesis. RANBP17 was first observed in the nuclei of round spermatids (steps 1-7) and then confined to the manchette of elongating spermatids (steps 8-14) together with its interacting partner SPEM1. In the Spem1-null testes, levels of RANBP17 were significantly elevated. As a member of a large protein family involved in the nucleocytoplasmic transport, RANBP17 may have a role in sex chromosome inactivation during the meiotic phase of spermatogenesis, and also in the intramanchette transport during spermiogenesis. Interactions between RANBP17 and SPEM1, for the first time, point to a potential function of SPEM1 in the RANBP17-mediated nucleocytoplasmic transport. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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