期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 317, 期 1-2, 页码 148-153出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2009.12.027
关键词
Insulin signal transduction; Insulin resistance; Berberine; L6 muscle cell
资金
- Hong Kong Jockey Club Charities Trust [JCICM-P2-05 CUHK]
Berberine has been shown to have insulin-sensitizing effect, but the molecular mechanism underlying remains elusive. In this work, we investigated the effect of berberine on insulin-induced signal transduction and glucose uptake in both insulin-sensitive and insulin-resistant rat skeletal muscle cells. Berberine increased the activity of AMPK and PKC zeta and AS 160 phosphorylation in normal cells, but had little effect on PKB activation. In insulin-resistant state, berberine exhibited synergistic effect on insulin-induced glucose uptake and GLUT4 translocation. Berberine improved insulin-induced tyrosine-phosphorylation of IRS-1 and the recruitment of p85 to IRS-1. These changes were accompanied by enhancement in insulin-induced PKC zeta and PKB activity and actin remodeling. The ameliorated insulin signal transduction was related to the inhibition of mTOR by berberine, which attenuated serine-phosphorylation of IRS-1. These results suggest that berberine may overcome insulin resistance via modulating key molecules in insulin signaling pathway, leading to increased glucose uptake in insulin-resistant cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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