期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 34, 期 19, 页码 3630-3641出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00243-14
关键词
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资金
- National Science Foundation of China [81230008, 91339114]
- Ministry of Education of People's Republic of China (111 project) [B07001]
- National Institute on Aging-IRP, National Institutes of Health
Methylation is a prevalent posttranscriptional modification of RNAs. However, whether mammalian microRNAs are methylated is unknown. Here, we show that the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo. Methylation by NSun2 inhibits the processing of pri-miR-125b2 into pre-miR-125b2, decreases the cleavage of pre-miR-125b2 into miR-125, and attenuates the recruitment of RISC by miR-125, thereby repressing the function of miR-125b in silencing gene expression. Our results highlight the impact of miR-125b function via methylation by NSun2.
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