期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 31, 期 1, 页码 226-236出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00821-10
关键词
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资金
- Breast Cancer Research Foundation
- National Institutes of Health [R01 CA18119, P01AG024387, T32ES007326, R01DK074967, P01CA8011105]
- DF/HCC Breast Cancer [P50C89393]
- U.S. Department of Defense Breast Cancer [W81XWH-08-1-0214]
- NATIONAL CANCER INSTITUTE [R01CA018119, P01CA080111] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK074967] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [T32ES007326] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P01AG024387] Funding Source: NIH RePORTER
The nuclear hormone receptor, estrogen receptor alpha (ER alpha), and mitogen-activated protein kinases (MAPKs) play key roles in hormone-dependent cancers, and yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ER alpha activates and interacts with extracellular signal-regulated kinase 2 (ERK2), a downstream effector in the MAPK pathway, resulting in ERK2 and ER alpha colocalization at chromatin binding sites across the genome of breast cancer cells. This genomic colocalization, predominantly at conserved distal enhancer sites, requires the activation of both ER alpha and ERK2 and enables ERK2 modulation of estrogen-dependent gene expression and proliferation programs. The ERK2 substrate CREB1 was also activated and recruited to ERK2-bound chromatin following estrogen treatment and found to cooperate with ER alpha/ERK2 in regulating gene transcription and cell cycle progression. Our study reveals a novel paradigm with convergence of ERK2 and ER alpha at the chromatin level that positions this kinase to support nuclear receptor activities in crucial and direct ways, a mode of collaboration likely to underlie MAPK regulation of gene expression by other nuclear receptors as well.
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