期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 29, 期 15, 页码 4295-4307出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00260-09
关键词
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资金
- ACS [RSG-05-01GMC, Searle 03-G107]
- CRCC [6-447140-34384]
- [R01GM069841]
- [R01HL077814]
- [DK42394]
- [HL52173]
- [PO1 HL057346]
The endoplasmic reticulum (ER) is the major cellular compartment where folding and maturation of secretory and membrane proteins take place. When protein folding needs exceed the capacity of the ER, the unfolded protein response (UPR) pathway modulates gene expression and downregulates protein translation to restore homeostasis. Here, we report that the UPR downregulates the synthesis of rRNA by inactivation of the RNA polymerase I basal transcription factor RRN3/TIF-IA. Inhibition of rRNA synthesis does not appear to involve the well-characterized mTOR (mammalian target of rapamycin) pathway; instead, PERK-dependent phosphorylation of eIF2 alpha plays a critical role in the inactivation of RRN3/TIF-IA. Downregulation of rRNA transcription occurs simultaneously or slightly prior to eIF2 alpha phosphorylation-induced translation repression. Since rRNA is the most abundant RNA species, constituting similar to 90% of total cellular RNA, its downregulation exerts a significant impact on cell physiology. Our study demonstrates the first link between regulation of translation and rRNA synthesis with phosphorylation of eIF2 alpha, suggesting that this pathway may be broadly utilized by stresses that activate eIF2 alpha kinases in order to coordinately regulate translation and ribosome biogenesis during cellular stress.
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