期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 29, 期 10, 页码 2594-2608出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01396-08
关键词
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资金
- Associazione Italiana per la Ricerca sul Cancro (AIRC)
- TRANSlational and Functional Onco-Genomics (TRANSFOG) [503438]
Recently, autophagy has emerged as a critical process in the control of T-cell homeostasis. Given the pivotal role of NF-kappa B in the signaling events of T cells, we have analyzed and unveiled a conserved NF-kappa B binding site in the promoter of the murine and human BECN1 autophagic gene (Atg6). Accordingly, we demonstrate that the NF-kappa B family member p65/RelA upregulates BECN1 mRNA and protein levels in different cellular systems. Moreover, p65-mediated upregulation of BECN1 is coupled to increased autophagy. The newly identified kappa B site in the BECN1 promoter specifically interacts with p65 both in vitro and in living Jurkat cells upon phorbol myristate acetate (PMA)-ionomycin stimulation, where p65 induction is coupled to BECN1 upregulation and autophagy induction. Finally, anti-CD3- and PMA-ionomycin-mediated activation of T-cell receptor signaling in peripheral T cells from lymph nodes of healthy mice results in an upregulation of BECN1 expression that can be blocked by the NF-kappa B inhibitor BAY 11-7082. Altogether, these data suggest that autophagy could represent a novel route modulated by p65 to regulate cell survival and control T-cell homeostasis.
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