期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 29, 期 1, 页码 150-156出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00997-08
关键词
-
资金
- MRC [G0300723, G0800784] Funding Source: UKRI
- Medical Research Council [G0800784B, G0300723B] Funding Source: researchfish
- Medical Research Council [G0300723, G0800784] Funding Source: Medline
We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains similar to 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据