期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 397, 期 1-2, 页码 245-253出版社
SPRINGER
DOI: 10.1007/s11010-014-2192-2
关键词
TNF-alpha; Apoptosis; Calcium; Antioxidants; Nanoparticles; U937 cells
类别
资金
- MICINN-FEDER [BFU 2010-15049]
- PRIN Project of the Italian Ministry of Education, University and Research (MIUR)
The present study is aimed to determine the protective effect of a novel nanoparticle with antioxidant properties, nanoceria, on reactive oxygen species (ROS) production, and calcium signaling evoked by the tumor necrosis factor-alpha (TNF alpha) in combination with cycloheximide (CHX) on apoptosis in the human histiocytic lymphoma cell line U937. Our results show that treatment of U937 cells with 10 ng/mL TNF alpha in combination with 1 mu g/mL CHX led to several Ca2+ alterations. These stimulatory effects on calcium signals were followed by intracellular ROS production and mitochondria membrane depolarization, as well as a time-dependent increase in caspase-8 and -9 activities. Our results show that the pretreatment with well known antioxidants such as trolox and N-acetyl cysteine (NAC) partially reduced the apoptotic effects due to the administration of TNF alpha plus cycloheximide. Furthermore, nanoceria had a stronger protective effect than trolox or NAC. Our findings also suggest that TNF alpha plus cycloheximide-induced apoptosis is dependent on alterations in cytosolic concentration of calcium [Ca2+](c) and ROS generation in human histiocytic U937 cells.
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