期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 369, 期 1-2, 页码 55-65出版社
SPRINGER
DOI: 10.1007/s11010-012-1368-x
关键词
Alzheimer's disease; PC 12 cells; A beta(25-35); Oxidative stress; Thymoquinone
类别
资金
- Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Ministry of Health and Family Welfare, Government of India, New Delhi
Beta-amyloid (A beta) peptides are considered to play a major role in the pathogenesis of Alzheimer's disease (AD) and compounds that can prevent pathways of A beta-induced neurotoxicity may be potential therapeutic agents for treatment of AD. This study examined the hypothesis that thymoquinone (TQ) would reduce oxidative stress and mitochondrial dysfunction in differentiated pheochromocytoma (PC 12) cells exposed to A beta fragment 25-35 (A beta(25-35)). To test this hypothesis, A beta was used to induce an in vitro model of AD in differentiated PC 12 cell line of rat. After 24 h of exposure with A beta(25-35), a significant reduction in cell viability and mitochondrial membrane potential (MMP) was observed. In addition, a significant elevation in the TBARS content and nitric oxide (NO) and activity of acetylcholine esterase (AChE) was observed which was restored significantly by TQ pretreatment. Furthermore, TQ also ameliorated glutathione and its dependent enzymes (glutathione peroxidase, glutathione reductase) which were depleted by A beta(25-35) in PC 12 cells. These results were supported by the immunocytochemical finding that has shown protection of cells by TQ from noxious effects of A beta(25-35). These results indicate that TQ holds potential for neuroprotection and may be a promising approach for the treatment of neurodegenerative disorders including AD.
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