期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 335, 期 1-2, 页码 83-89出版社
SPRINGER
DOI: 10.1007/s11010-009-0245-8
关键词
JNK; siRNA; CTGF; TGF-beta; Cornea
类别
资金
- Education Ministry Fund of China [20070422081]
- Natural Science Foundation of Shandong Province, China [2007GG20002031]
Transforming-growth factor-beta 1 (TGF-beta 1) plays an important role in fibrosis by inducing connective tissue growth factor (CTGF) expression. In cornea, however, signaling pathway in the process is unclear. In this paper, real-time RT-PCR and Western blot analysis were used to examine the expression and TGF-beta 1-induced upregulation of CTGF in telomerase-immortalized human cornea stroma fibroblast cells (THSFs). A detailed molecular mechanism for TGF-beta 1-mediated cell proliferation and collagen I production was then investigated by detecting the activation of JNK pathway in TGF-beta 1-stimulated THSFs and blocking the JNK pathway using JNK1/2 siRNA. Both TGF-beta 1 and CTGF clearly induced cell proliferation in THSFs which was inhibited by functional blocking CTGF by anti-CTGF antibody. Blocking JNK pathway by JNK1/2 siRNA significantly inhibited induction of CTGF by TGF-beta 1. Taken together, the data indicate that CTGF is critical to the fibrosis process in wounded human cornea, acting via upregulation and activation of JNK signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据