期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 193, 期 2, 页码 101-109出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2014.03.002
关键词
Apicomplexa; Malaria; Plasmodium berghei; Experimental genetics; P113; Liver stage
资金
- European Commission [PCIG10-GA-2011-304081, 34]
- Max Planck Society
Invasive stages of Plasmodium parasites possess distinct integral and peripheral membrane proteins that mediate host cell attachment and invasion. P113 is an abundant protein in detergent-resistant high molecular weight complexes in Plasmodium schizonts, but is unusual since expression extends to game-tocytes and sporozoites. In this study, we tested whether P113 performs important functions for parasite propagation in Plasmodium berghei. We show that pre-erythrocytic expression of P113 displays key signatures of upregulated in infectious sporozoites (UIS) genes, including control by the liver stage master regulator SLARP. Targeted gene deletion resulted in viable blood stage parasites that displayed no signs of blood stage growth defects. p113(-) parasites propagated normally through the life cycle until mature sporozoites, but displayed defects during natural sporozoite transmission, leading to a delay to patency in infected animals. By comparative in vitro and in vivo analysis of pre-erythrocytic development and using a xeno-diagnostic test we show that ablation of P113 results in lower sporozoite to liver stage conversion and, as a consequence, reduced merozoite output in vivo, without delaying liver stage development. We conclude that p113 is dispensable for Plasmodium life cycle progression and plays auxiliary roles during pre-erythrocytic development. (C) 2014 Elsevier B.V. All rights reserved.
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