期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 196, 期 1, 页码 29-40出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2014.07.011
关键词
PHIST; PRESAN; Plasmodium; Cytoskeleton; Protein export; Malaria
资金
- Wellcome Trust Grant [091095/Z/10/Z]
- Birkbeck College grant
- United Kingdom Medical Research Council [U117532063, U117532067]
- European Community's Seventh Framework Programme (FP7) [242095]
- Wellcome Trust [091095/Z/10/Z] Funding Source: Wellcome Trust
During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In P. falciparum, PHIST proteins have been implicated in protein trafficking and intercellular communication. A number of PHIST proteins are essential for parasite survival. Here, we identify nine members of the PHISTb sub-class of PHIST proteins, including one protein known to be essential for parasite survival, that localise to the erythrocyte periphery. These proteins have solubility characteristics consistent with their association with the erythrocyte cytoskeleton. Together, an extended PRESAN domain, comprising the PRESAN domain and preceding sequence, form a novel targeting-domain that is sufficient to localise a protein to the erythrocyte periphery. We validate the role of this domain in RESA, thus identifying a cytoskeleton-binding domain in RESA that functions independently of its known spectrin-binding domain. Our data suggest that some PHISTb proteins may act as cross-linkers of the erythrocyte cytoskeleton. We also show for the first time that peripherally-localised PHISTb proteins are encoded in genomes of P. knowlesi and vivax indicating a conserved role for the extended PRESAN domain of these proteins in targeting to the erythrocyte periphery. (C) 2014 The Authors. Published by Elsevier B.V.
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