期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 183, 期 2, 页码 158-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2012.02.013
关键词
Acanthamoeba; Encystation; Autophagy; Atg16
资金
- National Research Foundation of Korea (NRF)
- Korean government (MEST) [2010-0015585]
- Brain Korea 21 Project
Autophagy, an evolutionarily conserved protein degradation pathway in eukaryotes, plays essential roles during starvation and cellular differentiation by eliminating unwanted and/or unnecessary cell material including organelles. Autophagy protein 16 (Atg16) is an essential component of the autophagic machinery. The present study identified and characterized an Atg16 homologue (AcAtg16) in Acanthamoeba, an opportunistic pathogen responsible for several distinct diseases in humans. AcAtg16 was highly expressed during encystation and was found to be associated with small or large vesicular structures that partially colocalized with autophagolysosomes. Small interfering RNA against AcAtg16 inhibited autophagosome formation and reduced the encystation efficiency of Acanthamoeba. Moreover, most mitochondria remained undigested in these knockdown cells. Taken together, these results indicate that AcAtg16 is involved in autophagosome formation and plays an essential role in the encystation of Acanthamoeba. (C) 2012 Elsevier B.V. All rights reserved.
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