期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 176, 期 2, 页码 109-111出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2010.12.004
关键词
Leishmania donovani; Polyamines; Ornithine decarboxylase; Putrescine; alpha-Difluoromethylornithine
资金
- National Institute of Allergy and Infectious Diseases [R01 AI041622]
Administration of putrescine as a 1% solution in the drinking water ameliorated the profound loss of virulence exhibited by ornithine decarboxylase (ODC) deficient Leishmania donovani in mice. Furthermore, supplying alpha-difluoromethylornithine, an ODC inhibitor, at 2% in the drinking water reduced but did not eliminate infection with wild type L. donovani in the mouse model. Taken collectively, these findings: (1) demonstrate that oral putrescine can access the phagolysosome of macrophages in which the parasite resides in mice; (2) establish that the loss of virulence due to the Delta odc lesion is a consequence of the inability of the mutant parasite to synthesize sufficient polyamines de novo; (3) imply that the L donovani amastigote cannot access host polyamines in sufficient amounts for survival and growth; (4) and validate ODC as a drug target, although oral administration of DFMO is an unlikely therapeutic paradigm for visceral leishmaniasis. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据