期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 177, 期 1, 页码 61-64出版社
ELSEVIER
DOI: 10.1016/j.molbiopara.2011.01.010
关键词
NADP-linked isocitrate dehydrogenase; Trypanosomes; NADPH generation; Protection against ROS
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Universidad de Buenos Aires (UBA) [UBA-B094]
- Agencia Nacional de Promocion Cientifica y Tecnologica (Argentina)
- Universidad Nacional de General San Martin
Trypanosoma cruzi exhibits two putative isocitrate dehydrogenases (IDHs). Both idh genes were cloned and the recombinant enzymes expressed in Escherichia coli. Our results showed that T. cruzi IDHs are strictly dependent on NADP and display apparent affinities towards isocitrate and the coenzyme in the low micromolar range. In T. cruzi, IDHs are cytosolic and mitochondrial enzymes, and there is no evidence for the typical Krebs cycle-related NAD-dependent IDH. Hence, like in Trypanosoma brucei, the Krebs cycle is not a canonical route in T. cruzi. However, the citrate produced in the mitochondrion could be isomerized into isocitrate in the cytosol and the mitochondrion by means of the putative aconitase, which would provide the substrate for both IDHs. The cytosolic IDH is significantly more abundant in amastigotes, cell-derived and metacyclic trypomastigotes than in epimastigotes. This observation fits in well with the expected oxidative burst this pathogen has to face when infecting the mammalian host. (C) 2011 Elsevier B.V. All rights reserved.
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